Article
An alpha-tubulin mutant destabilizes the heterodimer: phenotypic consequences and interactions with tubulin-binding proteins.
Molecular biology of the cell - 1 Sept 1998
Vega L R, Fleming J, Solomon F
Abstract excerpt
Many effectors of microtubule assembly in vitro enhance the polymerization of subunits. However, several Saccharomyces cerevisiae genes that affect cellular microtubule-dependent processes appear to act at other steps in assembly and to affect polymerization only indirectly. Here we use a mutant alpha-tubulin to probe cellular regulation of microtubule assembly. tub1-724 mutant cells arrest at low temperature...
Topics
- Cold Temperature
- Dimerization
- Fungal Proteins
- Gene Expression
- Heterozygote
- Mutation
- Phenotype
- Precipitin Tests
- Protein Binding
- Schizosaccharomyces pombe Proteins
- Tubulin