Article
A movable and regulable inactivation function within the central region of a temperature-sensitive p53 mutant.
The Journal of biological chemistry - 13 Oct 1995
Chuang J Y, Lin C T, Wu C W, Lin Y S
Abstract excerpt
p53 is the most frequently mutated gene in human cancer. Naturally occurring mutations of p53 are mainly located within a region containing residues 100-300 and are predominantly of missense type, resulting in loss of the protein's DNA binding activity. Here we show that this type of mutation also represses the p53 N-terminal activation domain. The repression activity is localized in the central region of mutant...
Topics
- Animals
- Base Sequence
- Binding Sites
- CHO Cells
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Cricetinae
- DNA-Binding Proteins
- Fungal Proteins
- Genes, p53
- HeLa Cells