A temperature-sensitive mutant of human p53.

doi:10.1002/j.1460-2075.1994.tb06543.x

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Article

A temperature-sensitive mutant of human p53.

The EMBO journal - 1 Jun 1994

Zhang W, Guo X Y, Hu G Y, Liu W B, Shay J W, Deisseroth A B

Abstract excerpt

We discovered that the human 'hot-spot' p53 mutant 143Ala is temperature sensitive for the binding of two DNA elements and for mediating the transcription of a downstream luciferase reporter gene. At 32.5 degrees C, 143Ala possesses strong DNA binding ability. In addition, its transcriptional activities are stronger than those of wild-type p53. At 37.5 degrees C, however, both the mutant's DNA binding and...

Topics

Animals
Antibodies, Monoclonal
Cell Division
Cell Transformation, Neoplastic
DNA
Genes, Reporter
Genes, p53
Genes, ras
Humans
Mice
Mutation
Protein Binding

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Rats

Regulatory Sequences, Nucleic Acid

Structure-Activity Relationship

Temperature

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 8013454
DOI: 10.1002/j.1460-2075.1994.tb06543.x
PMCID: PMC395127

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8013454-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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