Article
Analysis of CD2 and TCR-beta gene expression in Jurkat cell mutants suggests a cis regulation of gene transcription.
Journal of immunology (Baltimore, Md. : 1950) - 15 Oct 1995
Kamoun M, Woods J S, Sano N, Makni H, Smith R, de Lau W B, van Oers A, Wotton D, Owen M J, Hashimoto Y, Clevers H C
Abstract excerpt
Thirty CD2- J32 stable clones, derived by mutagenesis and subsequent immunoselection with anti-CD2 Ab, were used to study the regulation of CD2 and TCR gene expression. Analysis of RNA expression revealed that the loss of surface expression of CD2 was due to a lack of expression of CD2 mRNA and was associated with a lack of expression of VDJ TCR-beta transcripts in 12 of these mutants, sparing the expression of...
Topics
- Antigenic Variation
- CD2 Antigens
- Cell Differentiation
- Gene Expression Regulation, Neoplastic
- Gene Transfer Techniques
- Genetic Complementation Test
- Humans
- In Situ Hybridization, Fluorescence
- Leukemia, T-Cell