Article
Random mutagenesis of the cAMP chemoattractant receptor, cAR1, of Dictyostelium. Mutant classes that cause discrete shifts in agonist affinity and lock the receptor in a novel activational intermediate.
The Journal of biological chemistry - 24 Jan 1997
Kim J Y, Caterina M J, Milne J L, Lin K C, Borleis J A, Devreotes P N
Abstract excerpt
The cAMP chemoattractant receptor, cAR1, of Dictyostelium transduces extracellular cAMP signals via G protein-dependent and G protein-independent mechanisms. While site-directed mutagenesis studies of G protein-coupled receptors have provided a host of information regarding the domains essential...
Topics
- Animals
- Dictyostelium
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins
- GTP-Binding Proteins
- Kinetics
- Mutagenesis
- Phenotype
- Phosphorylation
- Polymerase Chain Reaction
- Receptors, Cyclic AMP