Article
Multiplex DNA amplification and solid-phase direct sequencing for mutation analysis at the hprt locus in Chinese hamster cells.
Mutation research - 1 Aug 1993
Zu Z, Yu Y, Gibbs R A, Caskey C T, Hsie A W
Abstract excerpt
We report here the development of multiplex in vitro DNA amplification and solid-phase direct exon sequencing for the analysis of mutations at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus in Chinese hamster cells. 18 representative HPRT-deficient mutants, derived either spontaneously, or after exposure to UV light or ionizing radiation, were analyzed. All 9 hprt exons were simultaneously...
Topics
- Animals
- Base Sequence
- CHO Cells
- Chromosome Mapping
- Cricetinae
- DNA Mutational Analysis
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Sequence Analysis, DNA