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Article

Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase.

FEBS letters - 20 Nov 1998

Gudima S O, Kostyuk D A, Grishchenko O I, Tunitskaya V L, Memelova L V, Kochetkov S N

Abstract excerpt

Synthesis of deoxynucleotide-containing RNA-like single-stranded polynucleotides (dcRNAs) using the Y639F, S641A mutant of T7 RNA polymerase (T7 RNAP) was studied. A number of different T7 promoter-containing plasmids were tested as templates for dcRNA synthesis. The dcRNA synthesis efficiency strongly depended on the sequence of the first 8-10 nucleotides immediately downstream of the promoter and increased with...

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Identifiers

PMID: 9845342
DOI: 10.1016/s0014-5793(98)01393-3

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