Article
Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase.
FEBS letters - 20 Nov 1998
Gudima S O, Kostyuk D A, Grishchenko O I, Tunitskaya V L, Memelova L V, Kochetkov S N
Abstract excerpt
Synthesis of deoxynucleotide-containing RNA-like single-stranded polynucleotides (dcRNAs) using the Y639F, S641A mutant of T7 RNA polymerase (T7 RNAP) was studied. A number of different T7 promoter-containing plasmids were tested as templates for dcRNA synthesis. The dcRNA synthesis efficiency st...
Topics
- Bacteriophage T7
- DNA-Directed RNA Polymerases
- Deoxyribonucleotides
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Ribonucleotides
- Templates, Genetic
- Viral Proteins