Article
Mutations induced by dacarbazine activated with cytochrome P-450.
Mutation research - 1 Mar 1995
Mudipalli A, Nadadur S S, Maccubbin A E, Gurtoo H L
Abstract excerpt
The mutagenicity of the antitumor drug dacarbazine (DTIC) is due to alkylation of cellular DNA by metabolites resulting from the metabolism of this drug by the mixed function oxidase system. In the present study, we used an in vitro shuttle vector assay to study the base and sequence specificity...
Topics
- Biotransformation
- Cell Line, Transformed
- Cell-Free System
- Cytochrome P-450 Enzyme System
- Dacarbazine
- Escherichia coli
- Genes, Reporter
- Genes, Suppressor
- Genetic Vectors
- Humans
- Hydroxylation
- Kidney
- Methylation
- Microsomes, Liver
- Mutagenesis
- Mutation
- NADP
- Point Mutation