Article
Molecular analysis of lacI mutations in Rat2 cells exposed to 7,12-dimethylbenz[a]anthracene: evidence for DNA sequence and DNA strand biases for mutation.
Mutation research - 11 Nov 1996
Manjanatha M G, Chen J B, Shaddock J G, Harris A J, Shelton S D, Casciano D A
Abstract excerpt
The Rat2 cell line carries 50-70 stably integrated copies per cell of a lambda/lacI shuttle vector as a target for mutagenicity testing. Rat2 cells were exposed to 1 and 10 micrograms/ml of 7,12-dimethylbenz[a]anthracene (DMBA) for 24 h at 37 degrees C in the presence of primary rat hepatocytes,...
Topics
- 9,10-Dimethyl-1,2-benzanthracene
- Animals
- Animals, Genetically Modified
- Bacterial Proteins
- Bacteriophage lambda
- Carcinogens
- Cell Line
- Dose-Response Relationship, Drug
- Embryo, Mammalian
- Escherichia coli Proteins
- Lac Repressors
- Mutagenicity Tests
- Mutation
- Rats
- Repressor Proteins
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Transgenes