Article
Mutations induced in a shuttle vector plasmid exposed to monofunctionally activated mitomycin C.
Environmental and molecular mutagenesis - 1 Jan 1997
Maccubbin A E, Mudipalli A, Nadadur S S, Ersing N, Gurtoo H L
Abstract excerpt
Reductive activation of mitomycin C leads to its covalent binding to DNA, forming monoadducts and cross-links. The cytotoxicity of mitomycin C has been attributed to cross-link formation, whereas monoadducts are assumed to cause mutagenicity. We have developed a 32P-postlabeling technique to measure mitomycin C DNA adducts. Using this technique, we have measured monoadduct formation in the shuttle vector plasmid...
Topics
- Base Sequence
- Cell Line
- Genetic Vectors
- Humans
- Mitomycin
- Molecular Sequence Data
- Mutation
- Nucleic Acid Synthesis Inhibitors
- Plasmids