Article
Myelin basic protein peptide specificity and T-cell receptor gene usage of HPRT mutant T-cell clones in patients with multiple sclerosis.
Annals of neurology - 1 Nov 1994
Lodge P A, Allegretta M, Steinman L, Sriram S
Abstract excerpt
Characterization of T cells responding to autoantigens is central to understanding autoimmune disease. We have used somatic mutation at the hypoxanthine guanine phosphoribosyltransferase (HPRT) gene as an index of T-cell amplification in vivo. With this strategy we previously showed that myelin basic protein-reactive T cells can be isolated only from the HPRT mutant T-cell population cultured from the peripheral...
Topics
- Adult
- Amino Acid Sequence
- Base Sequence
- Clone Cells
- Epitopes
- Female
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Male
- Middle Aged
- Molecular Sequence Data