Molecular characterization of three loss-of-function mutations in the isopenicillin N-acyltransferase gene (penDE) of Penicillium chrysogenum.

Abstract excerpt

Five mutants of Penicillium chrysogenum blocked in penicillin biosynthesis (npe) which are deficient in isopenicillin N-acyltransferase were isolated previously. Three of these mutants, npe6, npe7, and npe8, have been characterized at the molecular level and compared with npe10, a deletion mutant. Transcripts of normal size (1.15 kb) of the penDE genes, which encode isopenicillin N-acyltransferase, and also of...

Topics

• Acyltransferases
• Amino Acid Sequence
• DNA, Bacterial
• Gene Expression
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• Penicillin-Binding Proteins
• Penicillium chrysogenum
• RNA, Bacterial