Article
Cloning and characterization of the isopenicillin N synthase gene of Streptomyces griseus NRRL 3851 and studies of expression and complementation of the cephamycin pathway in Streptomyces clavuligerus.
Antimicrobial agents and chemotherapy - 1 Jan 1991
García-Domínguez M, Liras P, Martín J F
Abstract excerpt
A gene, pcbC, encoding the isopenicillin N synthase of Streptomyces griseus NRRL 3851, has been cloned in a 6.4-kb Bg/II DNA fragment and located in an internal 1.55-kb PvuII segment by hybridization with the Penicillium chrysogenum pcbC gene. Hybridization studies revealed the presence of homolo...
Topics
- Actinomycetales
- Amino Acid Sequence
- Base Sequence
- Cell Line, Transformed
- Cephamycins
- Cloning, Molecular
- DNA
- Gene Expression
- Genetic Complementation Test
- Genomic Library
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oxidoreductases