Article
The isopenicillin-N acyltransferase of Penicillium chrysogenum has isopenicillin-N amidohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene.
European journal of biochemistry - 15 Jul 1993
Alvarez E, Meesschaert B, Montenegro E, Gutiérrez S, Díez B, Barredo J L, Martín J F
Abstract excerpt
The isopenicillin-N acyltransferase of Penicillium chrysogenum catalyzes the conversion of the biosynthetic intermediate isopenicillin N to the hydrophobic penicillins. The isopenicillin-N acyltransferase copurified with the acyl-CoA:6-aminopenicillanic acid (6-APA) acyltransferase activity which...
Topics
- Acyltransferases
- Amidohydrolases
- Amino Acid Sequence
- Culture Media
- Dithiothreitol
- Electrophoresis, Polyacrylamide Gel
- Enzyme Induction
- Gene Expression
- Molecular Sequence Data
- Mutation
- Penicillin Amidase
- Penicillin-Binding Proteins
- Penicillins