Article
A lacZ reporter fusion method for the genetic analysis of regulatory mutations in pathways of fungal secondary metabolism and its application to the Aspergillus nidulans penicillin pathway.
Journal of bacteriology - 1 Nov 1995
Pérez-Esteban B, Gómez-Pardo E, Peñalva M A
Abstract excerpt
Secondary metabolism, usually superfluous under laboratory conditions, is intrinsically elusive to genetic analysis of its regulation. We describe here a method of analyzing regulatory mutations affecting expression of secondary metabolic genes, with an Aspergillus nidulans penicillin structural...
Topics
- Aspergillus nidulans
- Crosses, Genetic
- Gene Expression
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genes, Recessive
- Genes, Reporter
- Genetic Linkage
- Lac Operon
- Molecular Biology
- Mutation
- Oxidoreductases
- Penicillins
- Selection, Genetic
- Transcription, Genetic
- beta-Galactosidase