Article
Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5.
Mutation research - 1 Jan 2000
Stackhouse M A, Ortiz J B, Sato K, Chen D J
Abstract excerpt
The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific...
Topics
- Animals
- Chromosomes, Human, Pair 5
- DNA Repair
- Genetic Complementation Test
- Humans
- In Situ Hybridization, Fluorescence
- Mice
- Mutation
- Polymerase Chain Reaction
- Radiation Tolerance
- Tumor Cells, Cultured