Article
Separation of origin recognition complex functions by cross-species complementation.
Science (New York, N.Y.) - 8 Dec 1995
Ehrenhofer-Murray A E, Gossen M, Pak D T, Botchan M R, Rine J
Abstract excerpt
Transcriptional silencing at the HMRa locus of Saccharomyces cerevisiae requires the function of the origin recognition complex (ORC), the replication initiator of yeast. Expression of a Drosophila melanogaster Orc2 complementary DNA in the yeast orc2-1 strain, which is defective for replication...
Topics
- Animals
- Cloning, Molecular
- DNA Replication
- DNA-Binding Proteins
- Drosophila Proteins
- Drosophila melanogaster
- Fungal Proteins
- Gene Expression Regulation
- Genes, Fungal
- Genes, Insect
- Genetic Complementation Test
- Mutation
- Origin Recognition Complex
- Regulatory Sequences, Nucleic Acid
- Replication Origin
- Repressor Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins