Article
Functional analysis of an Orc6 mutant in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America - 30 Jun 2009
Balasov Maxim, Huijbregts Richard P H, Chesnokov Igor
Abstract excerpt
The origin recognition complex (ORC) is a 6-subunit complex required for the initiation of DNA replication in eukaryotic organisms. ORC is also involved in other cell functions. The smallest Drosophila ORC subunit, Orc6, is important for both DNA replication and cytokinesis. To study the role of Orc6 in vivo, the orc6 gene was deleted by imprecise excision of P element. Lethal alleles of orc6 are defective in DNA...
Topics
- Animals
- Animals, Genetically Modified
- Cell Survival
- DNA Replication
- Drosophila
- Drosophila Proteins
- Drosophila melanogaster
- Fluorescent Antibody Technique
- G1 Phase
- Gene Deletion
- Genetic Complementation Test
- Green Fluorescent Proteins
- Humans
- Immunoblotting
- Immunohistochemistry
- Mitosis
- Mutation
- Neurons