Article
A targeted mutagenesis system for Actinobacillus pleuropneumoniae.
Gene - 7 Nov 1995
Mulks M H, Buysse J M
Abstract excerpt
We describe methods for the mutagenesis of cloned Actinobacillus pleuropneumoniae (Ap) genes and for the construction of Ap mutants by allelic exchange. We used these methods to construct isogenic mutants of Ap which no longer synthesized a 48-kDa outer membrane protein (AopA). The native aopA locus was replaced with a mutated locus that had been inactivated by insertion of a gene (KmR) encoding kanamycin...
Topics
- Actinobacillus pleuropneumoniae
- Alleles
- Bacterial Outer Membrane Proteins
- Mutagenesis