Construction of an Actinobacillus pleuropneumoniae-Escherichia coli cosmid cloning vector [Gene 160 (1995) 81-86].

doi:10.1016/0378-1119(95)00225-u

Back to search

Article

Construction of an Actinobacillus pleuropneumoniae-Escherichia coli cosmid cloning vector [Gene 160 (1995) 81-86].

Gene - 4 Jul 1995

West S E, Romero M J

Abstract excerpt

We constructed a cosmid vector, pSW206, which should be useful for the construction of genomic libraries of Actinobacillus pleuropneumoniae (Apl) DNA. pSW206 is based on the broad-host-range plasmid RK2 and can be introduced into Apl by conjugation.

Topics

Actinobacillus pleuropneumoniae
Cloning, Molecular
Conjugation, Genetic
Cosmids
DNA, Bacterial
Escherichia coli
Genetic Complementation Test
Genetic Vectors
Genomic Library
Phenotype
Plasmids
Restriction Mapping

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.