Article
Construction and characterization of a live, attenuated apxIICA inactivation mutant of Actinobacillus pleuropneumoniae lacking a drug resistance marker.
FEMS microbiology letters - 1 Feb 2005
Bei Weicheng, He Qigai, Yan Lin, Fang Liurong, Tan Yadi, Xiao Shaobo, Zhou Rui, Jin Meilin, Guo Aizhen, Lv Jianqiang, Huang Hongliang, Chen Huanchun
Abstract excerpt
The apxIIC gene of Actinobacillus pleuropneumoniae serotype 7 was inactivated by homologous recombination using a sucrose counter-selectable marker system, resulting in a mutant strain that had no antibiotic resistance marker and expressed an inactivated ApxII toxin. The safety and immunogenicity of the mutant were evaluated in mice. The mutant strain caused no adverse effects in mice at doses up to 2 x 10(9) CFU...
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