Article
Construction of isogenic mutants of Pasteurella haemolytica by allelic replacement.
Gene - 11 Oct 1994
Murphy G L, Whitworth L C
Abstract excerpt
We describe methods for the mutagenesis of cloned Pasteurella haemolytica (Gram-) genes and for the construction of P. haemolytica mutants by allelic exchange. We used these methods to construct isogenic mutants of P. haemolytica which no longer synthesize three membrane lipoproteins (Lpp). A sin...
Topics
- Alleles
- Crossing Over, Genetic
- DNA, Bacterial
- Electroporation
- Gene Dosage
- Genes, Bacterial
- Lipoproteins
- Mannheimia haemolytica
- Membrane Proteins
- Mutagenesis
- Plasmids
- Transformation, Bacterial
- beta-Lactamases