Article
Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes.
Gene - 4 Jul 1995
West S E, Romero M J, Regassa L B, Zielinski N A, Welch R A
Abstract excerpt
We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZ alpha-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
