Article
[Generation of nalidixic acid-resistant strains and signature-tagged mutants of Actinobacillus pleuropneumoniae].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Jan 2008
Shang Lin, Li Wei, Li Liangjun, Li Lu, Zhang Sihua, Li Tingting, Li Yaokun, Liu Lei, Guo Zhiwei, Zhou Rui, Chen Huanchun
Abstract excerpt
Actinobacillus pleuropneumoniae is a very important respiratory pathogen for swine and causes great economic losses in pig industry worldwide. Signature-tagged mutagenesis (STM) is an effective method to identify virulence genes in bacteria. In this study, we selected nalidixic acid-resistant strains of APP serotypes 1 and 3 by in vitro cultivation, and used as receipt strains for constructing transposon mutants...
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