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Article

Harnessing natural transformation in Actinobacillus pleuropneumoniae: a simple method for allelic replacements.

FEMS microbiology letters - 15 Apr 2004

Bossé Janine T, Nash John H E, Kroll J Simon, Langford Paul R

Abstract excerpt

We have investigated the use of a natural transformation protocol to introduce mutations into Actinobacillus pleuropneumoniae serotypes 1 and 5b. For both strains tested, we recovered 1 in 10(8) transformants during culture in rich medium, a result that was independent of the growth phase. This low frequency of transformation of A. pleuropneumoniae did not increase when bacteria were grown under conditions known...

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PMID: 15063496
DOI: 10.1016/j.femsle.2004.02.022

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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