Article
Actinobacillus pleuropneumoniae serotype 1 carrying the defined aroA mutation is fully avirulent in the pig.
Research in veterinary science - 1 Apr 2002
Garside L H, Collins M, Langford P R, Rycroft A N
Abstract excerpt
The aroA gene from Actinobacillus pleuropneumoniae serotype 1 reference strain 4074 was isolated and sequenced. The gene complemented the aroA mutation in Escherichia coli AB2829. A kanamycin resistance cassette was inserted into the aroA gene and the mutant gene was reintroduced into A. pleuropneumoniae by allelic replacement. Intratracheal infection of susceptible pigs with A. pleuropneumoniae aroA caused no...
Topics
- 3-Phosphoshikimate 1-Carboxyvinyltransferase
- Actinobacillus Infections
- Actinobacillus pleuropneumoniae
- Alkyl and Aryl Transferases
- Alleles
- Animals
- Gene Expression
- Gene Transfer Techniques
- Kanamycin
- Kanamycin Resistance