Article
A comprehensive approach for typing the alleles of the HLA-B locus by automated sequencing.
Tissue antigens - 1 Aug 1995
Petersdorf E W, Hansen J A
Abstract excerpt
We describe an approach for typing alleles of the HLA-B locus by using automated sequencing technology. The exon 2 and exon 3 nucleotide sequence of each allele is determined directly from genomic DNA in two steps. In the first step, HLA-B exon 2, intron 2 and exon 3 sequences are amplified with one or two primer pairs out of a panel of 5 primer pairs that describe all known HLA-B alleles. In the second step,...
Topics
- Alleles
- Base Sequence
- DNA Primers
- Epitopes
- HLA-B Antigens
- HLA-B15 Antigen
- HLA-B27 Antigen
- HLA-B39 Antigen
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction