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Article

Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA.

Journal of immunotherapy (Hagerstown, Md. : 1997) - 1 Nov 1997

Bettinotti M P, Mitsuishi Y, Bibee K, Lau M, Terasaki P I

Abstract excerpt

Molecular testing is gradually replacing standard typing techniques in the field of HLA because it allows higher resolution, which has significant functional implications. Although several techniques have been so far described for this purpose, the definitive means to determine which alleles are present in a particular sample is to identify their sequence. We describe a simplified method for typing HLA-A, B, and...

Topics

Alleles
DNA
HLA-A Antigens
HLA-B Antigens
HLA-C Antigens
Histocompatibility Testing
Humans
Polymerase Chain Reaction
Sequence Analysis, DNA
Templates, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 9409447
DOI: 10.1097/00002371-199711000-00001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-9409447-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA.PMID 9409447Read ↗

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