HLA-B typing by allele separation followed by direct sequencing.

doi:10.1111/j.1399-0039.1997.tb02764.x

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Article

HLA-B typing by allele separation followed by direct sequencing.

Tissue antigens - 1 Apr 1997

Eberle M, Knapp L A, Iwanaga K K, Domanico M J, Aiyer K, Watkins D I

Abstract excerpt

Due to the enormous allelic diversity of the HLA-B locus, it has been difficult to design an unambiguous molecular typing method for the alleles at this locus. Here we describe a technique for the direct sequencing of HLA-B alleles. Initially, HLA-B alleles were PCR-amplified after locus-specific...

Topics

Alleles
HLA-B Antigens
Histocompatibility Testing
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA

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