Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

HLA-B typing by allele separation followed by direct sequencing.

Tissue antigens - 1 Apr 1997

Eberle M, Knapp L A, Iwanaga K K, Domanico M J, Aiyer K, Watkins D I

Abstract excerpt

Due to the enormous allelic diversity of the HLA-B locus, it has been difficult to design an unambiguous molecular typing method for the alleles at this locus. Here we describe a technique for the direct sequencing of HLA-B alleles. Initially, HLA-B alleles were PCR-amplified after locus-specific reverse transcription of RNA. Alleles were then separated using denaturing gradient gel electrophoresis (DGGE), which...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Histocompatibility Testing
Share to HLA-B Antigens

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Molecular Sequence Data

Share to Polymerase Chain Reaction

Share to Sequence Analysis, DNA

Source

Identifiers

PMID: 9151388
DOI: 10.1111/j.1399-0039.1997.tb02764.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

HLA-B typing by allele separation followed by direct sequencing.PMID 9151388Read ↗

Full screening and accurate subtyping of HLA-A*02 alleles through group-specific amplification and mono-allelic sequencing.Semantic proximity Read ↗

Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA.Semantic proximity Read ↗

Human leukocyte antigen (HLA) typing by DNA sequencing.Semantic proximity Read ↗

HLA-DRB1 DNA sequencing based typing: an approach suitable for high throughput typing including unrelated bone marrow registry donors.Semantic proximity Read ↗

High-resolution HLA-DRB typing using denaturing gradient gel electrophoresis and direct sequencing.Semantic proximity Read ↗

HLA-B and HLA-C Supratyping by Pyrosequencing®.Semantic proximity Read ↗

A comprehensive approach for typing the alleles of the HLA-B locus by automated sequencing.Semantic proximity Read ↗

HLA-DQB1 sequencing-based typing using newly identified conserved nucleotide sequences in introns 1 and 2.Semantic proximity Read ↗

Complete subtyping of the HLA-A locus by sequence-specific amplification followed by direct sequencing or single-strand conformation polymorphism analysis.Semantic proximity Read ↗

HLA class I sequence-based typing using DNA recovered from frozen plasma.Semantic proximity Read ↗

Cloning and sequencing full-length HLA-B and -C genes.Semantic proximity Read ↗

HLA class I sequence-based typing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.