Article
Low-cost, simultaneous, single-sequence genotyping of the HLA-A, HLA-B and HLA-C loci.
Tissue antigens - 1 Jul 2006
Abbott W G H, Tukuitonga C F, Ofanoa M, Munn S R, Gane E J
Abstract excerpt
New automated DNA sequencing technology has enabled the development of an assay for genotyping the three major HLA class 1 loci from a single sequence of each gene containing exon 3, intron 2 and exon 2. The assay allows 31 subjects (with 3 negative controls) to be genotyped at all three loci simultaneously, using a 96-well plate format. Genotypes were assigned by comparing each sequence to a database of 307...
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