Article
A strategy for high throughput HLA-DQ typing.
Journal of immunological methods - 1 Dec 2001
Feolo M, Fuller T C, Taylor M, Zone J J, Neuhausen S L
Abstract excerpt
We have developed a high throughput HLA typing methodology that is a modification of the standard sequence-specific primer method. This approach is distinct from other methods using an automated DNA analyzer, as more than one gene is typed in a single lane. We have optimized the method for use on an ABI 373 automated genotyping machine. Primers were designed to preferentially amplify DNA fragments of the generic...
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