Article
Cloning and sequencing full-length HLA-B and -C genes.
Tissue antigens - 1 Jan 2003
Cox S T, McWhinnie A J, Robinson J, Marsh S G E, Parham P, Madrigal J A, Little A-M
Abstract excerpt
Currently most available HLA-A, -B and -C DNA sequences cover exons 2 and 3 with a limited number extending to include other exons and introns. We have developed a method for the accurate determination of full-length genomic DNA sequences for HLA-A, -B and -C alleles. The method involves cloning of PCR amplified full-length HLA genes to separate alleles at heterozygous loci. The approach avoids any ambiguities...
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