DNA binding contributes to dominant-negative effects of a catalytically inactive RecQ4-family helicase.

Abstract excerpt

DNA inter-strand crosslinks (ICLs) are highly cytotoxic lesions that require coordinated processing for repair. The RecQ4-family helicase Hrq1 promotes ICL repair in Saccharomyces cerevisiae, yet the mechanistic basis of its function remains unclear. Notably, the catalytically inactive hrq1-K318A allele confers greater sensitivity to ICL-inducing agents than deletion of HRQ1, suggesting a dominant-negative...

Topics

• RecQ Helicases
• Saccharomyces cerevisiae Proteins
• Saccharomyces cerevisiae
• DNA Repair
• Protein Binding
• Mutation