Article
Phenotypic screening for small molecules that lower PrP in cultured cells.
Molecular and cellular neurosciences - 1 Sept 2026
Frei Jeannine A, Reidenbach Andrew G, Xu Leo M H, Gopalakrishnan Raja Mohan, Casalana Dominick, Sprague Daniel A, Bray Mark-Anthony, Wang Amy Q, Laversenne Vanessa, Erickson Brian, Braun Craig, Hall Mckenzie, Auld Douglas, Minikel Eric Vallabh, Vallabh Sonia M
Abstract excerpt
Prion protein (PrP) lowering is a validated therapeutic hypothesis in prion disease. To identify small molecules that reduce PrP levels, we performed phenotypic screening in cultured cells. To prioritize PrP specificity in our primary screen, we generated mouse N2a cells stably expressing GFP and used high content imaging analysis to select compounds that lowered PrP without affecting GFP signal or cell...
Read the complete abstract on PubMedTopics
- Animals
- Mice
- Small Molecule Libraries
- Prion Proteins
- Prions