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Article

Protocol for first-hit oncogenic organoid modeling and lentiviral functional screening.

STAR protocols - 19 Jun 2026

Yousef Farah, Mosley Jaramys, Karginova Olga, Seoane Jose A, Salahudeen Ameen A

Abstract excerpt

We present a protocol for modeling early tumorigenesis using air-liquid interface (ALI) organoids derived from oral, esophageal, and lung epithelia bearing first-hit oncogenic mutations. We describe steps for identifying candidate amplified oncogenes through outlier analysis, establishing ALI organoids, and inducing oncogenic activation. We then perform pooled lentiviral open reading frame (ORF) library delivery,...

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Identifiers

PMID: 42284149
DOI: 10.1016/j.xpro.2026.104625
PMCID: PMC13277434

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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