Article
CRISPR/dCas9-Mediated Specific Molecular Assembly Facilitates Genotyping of Mutant Circulating Tumor DNA.
Analytical chemistry - 7 Nov 2023
Liang Tingting, Qin Xiaojie, Zhang Yuyuan, Yang Yu, Chen Yu, Yuan Lin, Liu Feng, Chen Zhizhong, Li Xinchun, Yang Fan
Abstract excerpt
Breakthroughs in circulating tumor DNA (ctDNA) analysis are critical in tumor liquid biopsies but remain a technical challenge due to the double-stranded structure, extremely low abundance, and short half-life of ctDNA. Here, we report an electrochemical CRISPR/dCas9 sensor (E-dCas9) for sensitive and specific detection of ctDNA at a single-nucleotide resolution. The E-dCas9 design harnesses the specific capture...
Topics
- Humans
- Circulating Tumor DNA
- Carcinoma, Non-Small-Cell Lung
- Lung Neoplasms
- Clustered Regularly Interspaced Short Palindromic Repeats
- Genotype
- Biomarkers, Tumor
- Mutation