Article
Mutations that confer resistance to sodium azide affect the levels of two physiological isoforms of SecA in Escherichia coli.
Microbiology (Reading, England) - 1 May 2026
Jiang Chen, Milner Mathew, Williams Georgia, Abdullah Osama, Lund Peter, Huber Damon
Abstract excerpt
Sodium azide inhibits bacterial growth by inhibiting the SecA-dependent translocation of proteins across the cytoplasmic membrane, and all of the mutations known to confer increased resistance to azide are in the secA gene. However, the molecular mechanism of resistance is unclear. To gain insight into this mechanism, we designed a genetic screen to isolate transposon insertion mutations that conferred increased...
Topics
- Escherichia coli
- Escherichia coli Proteins
- Protein Isoforms
- Mutation
- SEC Translocation Channels
- SecA Proteins
- Membrane Transport Proteins
- Sodium Azide
- Bacterial Proteins
- Drug Resistance, Bacterial
- Adenosine Triphosphatases
- Mutagenesis, Insertional