Article
Isolation and analysis of dominant secA mutations in Escherichia coli.
Journal of bacteriology - 1 Jan 1991
Jarosik G P, Oliver D B
Abstract excerpt
The secA gene product is an autoregulated, membrane-associated ATPase which catalyzes protein export across the Escherichia coli plasma membrane. Previous genetic selective strategies have yielded secA mutations at a limited number of sites. In order to define additional regions of the SecA protein that are important in its biological function, we mutagenized a plasmid-encoded copy of the secA gene to create...
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