Article
Analysis of Mutagenesis in the Escherichia coli tdk Gene: The Nucleoid Protein HNS Is Required for a Dramatic Cisplatin-Induced Hotspot.
Environmental and molecular mutagenesis - 1 Apr 2026
Sorensen Dana, Mata Tara, Sridharan Ananya, Mathew Mallika, Sagastume Emily, Sutkin Natasha, Douglas Katherine, Daniel Mila, Hung Angela, Garmash Alina, Hsieh Annette, Ortega Jamie Elizabeth, Miller Jeffrey H
Abstract excerpt
We demonstrate the utility of the tdk reporter gene system, by showing its ability to not only analyze mutational hotspots, but also to allow the analysis of both weak and strong mutator or mutagen effects. It can detect large and small insertions and deletions, as well as base substitutions. We previously defined cisplatin (CPT)-induced hotspots in tdk. One extraordinary hotspot for G:C- > T:A transversions at...
Topics
- Cisplatin
- Escherichia coli Proteins
- Escherichia coli
- DNA-Binding Proteins
- Mutagenesis
- Mutation
- DNA, Bacterial
- Genes, Reporter