Article
Enhanced characterization of the thyA system for mutational analysis in Escherichia coli: Defining mutationally "hot" regions of the gene.
Mutation research - 1 Jan 2000
Mashiach Daniel, Bacasen Erin Mae, Singh Sunjum, Kao Timothy, Yaramada Lekha, Mishail Daniel, Singh Summer, Miller Jeffrey H
Abstract excerpt
We have extensively characterized base substitution mutations in the 795 base pair (bp) long E. coli thyA gene to define as many of the base substitution mutational sites that inactivate the gene as possible. The resulting catalog of mutational sites constitutes a system with up to 5 times as many sites for monitoring each of the six base substitution mutations as the widely used rpoB/Rifr system. We have defined...
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