Article
Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-Associated Proteins in Escherichia coli.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Schnetz Karin
Abstract excerpt
Bacterial nucleoid-associated proteins are important in nucleoid-structuring, homeostasis of DNA supercoiling, and in regulation of transcription. Vice versa, transcription influences DNA supercoiling and possibly DNA-binding of nucleoid-associated proteins. Here, I describe genetic tools to study the interplay between transcription and nucleoid-associated proteins such as H-NS in Escherichia coli. These standard...
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