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Engineering Escherichia coli for the High-Titer Biosynthesis of Lacto-N-tetraose

22 Jun 2022

Abstract excerpt

Lacto- N -tetraose (LNT), a member of the human milk oligosaccharides family, has received widespread attention because of its importance in infant health. We constructed a whole-cell biotransformation method in Escherichia coli BL21(DE3) for high-titer LNT synthesis. The approach was performed by using a systematic design and metabolic engineering based on the metabolic pathway of LNT. The lgtA (encoding β-1,3-...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 35731707
DOI: 10.1021/acs.jafc.2c02423

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