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Article

Enhancing the catalytic activity of a GH5 processive endoglucanase from Bacillus subtilis BS-5 by site-directed mutagenesis.

International journal of biological macromolecules - 31 Jan 2021

Lv Kemin, Shao Wenyu, Pedroso Marcelo Monteiro, Peng Jiayu, Wu Bin, Li Jiahuang, He Bingfang, Schenk Gerhard

Abstract excerpt

Processive endoglucanases possess both endo- and exoglucanase activity, making them attractive discovery and engineering targets. Here, a processive endoglucanase EG5C-1 from Bacillus subtilis was employed as the starting point for enzyme engineering. Referring to the complex structure information of EG5C-1 and cellohexaose, the amino acid residues in the active site architecture were identified and subjected to...

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Identifiers

PMID: 33310097
DOI: 10.1016/j.ijbiomac.2020.12.060

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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