Article
Engineering of β-Glucosidase Bgl15 with Simultaneously Enhanced Glucose Tolerance and Thermostability To Improve Its Performance in High-Solid Cellulose Hydrolysis.
Journal of agricultural and food chemistry - 13 May 2020
Cao Lichuang, Chen Ran, Huang Xin, Li Shuifeng, Zhang Sufang, Yang Xiangpeng, Qin Zongmin, Kong Wei, Xie Wei, Liu Yuhuan
Abstract excerpt
In this study, a Petri-dish-based double-layer high-throughput screening method was established to improve glucose tolerance of β-glucosidase Bgl15. Two beneficial mutations were identified, and the joint mutant 2R1 improved the half-maximal inhibitory concentration of glucose from 0.04 to 2.1 M. The crystal structure of 2R1 was subsequently determined at 2.7 Å. Structure analysis revealed that enhancement of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.