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Article

Design and application of a lactulose biosensor.

Scientific reports - 7 Apr 2017

Wu Jieyuan, Jiang Peixia, Chen Wei, Xiong Dandan, Huang Linglan, Jia Junying, Chen Yuanyuan, Jin Jian-Ming, Tang Shuang-Yan

Abstract excerpt

In this study the repressor of Escherichia coli lac operon, LacI, has been engineered for altered effector specificity. A LacI saturation mutagenesis library was subjected to Fluorescence Activated Cell Sorting (FACS) dual screening. Mutant LacI-L5 was selected and it is specifically induced by lactulose but not by other disaccharides tested (lactose, epilactose, maltose, sucrose, cellobiose and melibiose)....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28387245
DOI: 10.1038/srep45994
PMCID: PMC5384092

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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