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Article

Deep-mutational scanning libraries using Tiled-Region Exchange mutagenesis.

G3 (Bethesda, Md.) - 4 Mar 2026

Kindree Kortni, Chochinov Claire A, Bhachu Keerath, Cheng Yunyi, Caron Amelia, McDonald Molly, Mamai Zaynab, Nguyen Ba Alex N

Abstract excerpt

The analysis of gene function frequently requires the generation of mutants. Deep-mutational scanning (DMS) has emerged as a powerful tool to decipher important functional residues within genes and proteins. However, methods for performing DMS tend to be complex or laborious. Here, we introduce Tiled-Region Exchange (T-REx) mutagenesis, which is a multiplexed modification of the Extremely Methodical and Parallel...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41530905
DOI: 10.1093/g3journal/jkag006
PMCID: PMC12958811

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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