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RLB-MALBAC: A rapid, non-transposase method for synthesizing unbiased sequencing libraries from low amount of genomic DNA for Nanopore sequencing

2024-12-05

Abstract excerpt

<title>Abstract</title> <p><bold>Background</bold> Successful sequencing of genomes on Oxford Nanopore Technologies (ONT) sequencing platforms is dependant on obtaining sufficient amounts of high-quality genomic DNA. ONT’s Rapid PCR kit (SQK-RPB114.24) kit is specifically designed to address input template limitations, whereby low amounts of target DNA (1–5 ng) is pre-amplified to > 100–200 ng using PCR. The succ...

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Literature Corpus work: c3827e0b-de68-5c72-a34c-e1c88e330411
DOI: 10.21203/rs.3.rs-5141136/v1

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