Article
Nucleic acid detection with CRISPR-Cas13a/C2c2.
Science (New York, N.Y.) - 28 Apr 2017
Gootenberg Jonathan S, Abudayyeh Omar O, Lee Jeong Wook, Essletzbichler Patrick, Dy Aaron J, Joung Julia, Verdine Vanessa, Donghia Nina, Daringer Nichole M, Freije Catherine A, Myhrvold Cameron, Bhattacharyya Roby P, Livny Jonathan, Regev Aviv, Koonin Eugene V, Hung Deborah T, Sabeti Pardis C, Collins James J, Zhang Feng
Abstract excerpt
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with...
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