Article
Two-step computational redesign of Bacillus subtilis cellulase and β-glucanase for enhanced thermostability and activity.
International journal of biological macromolecules - 1 Jan 2025
Zhang Huan, Zhu Tong, Zhai Qinglin, Chen Qiansi, Zhang Xuanshuo, Chen Yiqiang, He Wei, Li Jingjing, Fan Jianqiang, Tao Jiemeng, Hu Xingchuan, Qi Lingfeng, Wang Chaochao, Liao Kuanqi, Chen Yanchun, Cui Yinglu, Chen Shanyi, Wu Bian
Abstract excerpt
The growing demand for biocatalysts in biomass processing highlights the necessity of enhancing the thermostability of glycoside hydrolases. However, improving both thermostability and activity is often hindered by trade-offs between backbone rigidity and the flexibility of substrate-binding regions. In this study, Bacillus subtilis cellulase and β-glucanase were engineered using a two-step process incorporating...
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