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Article

Interface-Targeted Rational Design Strategies for Enhancing the Thermostability of d-Allulose 3-Epimerase.

Journal of agricultural and food chemistry - 29 Jul 2026

Qiu Kaifan, Li Xingfei, Bai Yuxiang, Li Xiaoxiao, Jin Zhengyu, Long Jie

Abstract excerpt

d-Allulose 3-epimerase (DAEase) catalyzes d-fructose conversion to d-allulose, but the poor thermostability of Clostridium cellulolyticumH10 DAEase limits its industrial application. Here, we enhanced DAEase thermostability by targeting the subunit interface using PROSS-guided combinatorial engineering and spatial clustering. Candidate mutations were classified into interface core, interface-adjacent, and distal...

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Carbohydrate Epimerases
Enzyme Stability
Fructose
Hot Temperature
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Molecular

Mutagenesis, Site-Directed

Protein Engineering

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PMID: 42448649
DOI: 10.1021/acs.jafc.6c05893

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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